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Recombinant endo-α-N-acetylgalactosaminidase from Bifidobacterium longum, E. coli

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Cat. No.: X24-08-LY325

Recombinant endo-α-N-acetylgalactosaminidase from Bifidobacterium longum, E. coli

Synonym: Endo-α

MDL: MFCD00131003
CAS Number: 59793-96-3
Enzyme Commission Number: 3.2.1.97

Product Size

1 Vial (100m units)

Price

Datasheet

MSDS

Properties

Description

Recombinant endo-α-N-acetylgalactosaminidase from Bifidobacterium longum is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group.

Unit Definition

One unit will produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP per minute at pH 6.0 at 37°C.

Expression System

E. coli

Source

Bifidobacterium longum

Reaction Conditions

50 mM Sodium acetate, pH 5.0

Production Methods

Fermentation

Detection Method

The 20 μL mixture of recombinant endo-α-N-acetylgalactosaminidase and Galβ1-3GalNAc α-pNP is incubated at 37°C for 10 min. After waiting for the reaction to finish, 30 μL of 0.25 M sodium carbonate is added for bursting. Importantly, the quantification of released pNP is measured by UV spectroscopy.

Sensitivity

Heat sensitive

Concentration

100 munits/vial

Form

White powder to crystal

Titer

80-120 mU/vial

Preservative

Stabilizer

Applications

Recombinant endo-α-N-acetylgalactosaminidase from Bifidobacterium longum can be used to produce Galβ1-3GalNAc (galacto-N-biose) by hydrolysis.

Publications

Publications (0)

Publications

Publications

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