Recombinant Bacillus subtilis levanase 32A produced by Escherichia coli, breaks down the 2,6-β-D-fructofuranosidic linkages found in 2,6-β-D-fructans (levans) comprising more than 3 fructose units.
Expression System
Escherichia coli
Species
Bacillus subtilis
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Levanases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis levanase 32A can be used for glycobiology research. It has the ability to hydrolyze levans.
Publications
Publications (0)
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Publications
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