Recombinant Bacteroides uniformis β-agarase 86A produced by Escherichia coli, is responsible for cleaving the core of agarose polysaccharides into new agar oligosaccharides.
Expression System
Escherichia coli
Species
Bacteroides uniformis
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Agarases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides uniformis β-agarase 86A can be used to recover DNA or RNA fragments from low-melting-point agarose for amplification, cloning, or sequencing. It has the ability to hydrolyze agarose and porphyrin.
Publications
Publications (0)
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Publications
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