Properties
Description
Recombinant Clostridium thermocellum rhamnogalacturonan lyase 11A produced by Escherichia coli, enzymatically cleaves the α-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acid residues in type I rhamnogalacturonan (RG-I) through a β-elimination reaction.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Rhamnogalacturonan lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum rhamnogalacturonan lyase 11A can be used for glycobiology research and participates in the eliminative cleavage of rhamnogalacturonans.
