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Recombinant Flavobacterium meningosept-icum peptide-N-glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins), E. coli
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Cat. No.: X23-03-YW092

Recombinant Flavobacterium meningosept-icum peptide-N-glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins), E. coli

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Synonym: PNGaseF

  • Enzyme Commission Number: 3.5.1.52
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Product Size
50 U; 100 U; 250 U; 500 U; 3 KU
Price
Datasheet
Datasheet
MSDS
MSDS
Properties
Description
It is a recombinant Flavobacterium meningosept-icum peptide-N-glycosidase F (high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins) produced in E. coli. This product can be used to study the mode of action of the enzyme and its potential inhibitors, and can also be used as a glycoengineering tool to modify glycoprotein in vitro.
Unit Definition
One unit will catalyze the removal >95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 µl in one hour at 37°C.
Source
Flavobacterium meningosept-icum
Form
Lyophilized powder
Applications
It can cleave high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins and glycopeptides between the innermost GlcNAc and asparagine residues. As a consequence, a free glycan and a deaminated protein or peptide are produced.
Safety Information
Personal Protective Equipment
Gloves, eyeshields, N95 mask
Publications
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