Recombinant Lactococcus lactis phospho-β-galactosidase 1A produced by Escherichia coli, has the E.C. number 3.2.1.86 and is a 6-phospho-β-glucosidase enzyme.
Expression System
Escherichia coli
Species
Lactococcus lactis
Concentration
1 mg/mL
Form
Liquid
Purity
≥90%, determined by SDS-PAGE.
Activity
Phospho-β-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Lactococcus lactis phospho-β-galactosidase 1A can be used in carbohydrate and biofuel research. It has the ability to hydrolyze O-nitrophenyl P-D-galactopyranoside (ONPG), and o-nitrophenyl P-galactopyranoside 6-phosphate (ONPG-6P7).
Publications
Publications (0)
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Publications
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