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Recombinant Oryza sativa (rice) PNGase A, Pichia pastoris

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Cat. No.: X24-08-LY317

Recombinant Oryza sativa (rice) PNGase A, Pichia pastoris

Name: Recombinant Oryza sativa (rice) PNGase A, Pichia pastoris
SKU: X24-08-LY317

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Synonym: PNGase A

Product Size

150 U; 300 U; 450 U; 600 U; 750 U

Price

Datasheet

Datasheet

MSDS

MSDS

Properties

Description

PNGase A is a recombinant amidase derived from Oryza sativa and expressed in Pichia pastoris. It cleaves N-linked glycans with or without α(1,3)-linked core fucose residues.

Molecular Weight

63.8 kDa

Unit Definition

One unit will remove > 95% of the carbohydrate from 1 µg of denatured recombinant Avidin produced in Maize in 1 hour at 37°C in a total reaction volume of 10 µl.

Expression System

Pichia pastoris

Source

Oryza sativa

Components

PNGase A (5,000 units/ml); 10 x GlycoBuffer (50 mM sodium acetate, pH 6, 25°C); Glycoprotein denaturing buffer (10 x); NP-40 (10%)

Reaction Conditions

1X GlycoBuffer (50 mM sodium acetate, pH 6, 25°C), Incubate at 37°C

Heat Inactivation

10 minutes at 65°C

Production Methods

Fermentation

Detection Method

One microgram of recombinant avidin undergoes denaturation in 1X glycoprotein denaturing buffer at 100°C for 10 minutes.. Subsequent to this, NP-40 and GlycoBuffer are introduced into the mixture. Thereafter, two-fold dilutions of PNGase A are added, and the reaction mixture is incubated at 37°C for one hour. The separation and visualization of the reaction products are analyzed by SDS-PAGE.

Concentration

5,000 units/ml

Form

Powder

Purity

≥95%

Buffer

20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5 at 25°C

Applications

PNGase A can be used to cleave high mannose N-glycan structures from Man 3 up to Man 9.

Publications

Publications (0)

Publications

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Publications

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