Properties
Description
Recombinant Pseudoalteromonas sp. ulvan lyase 24A produced by Escherichia coli, catalyzes the eliminative cleavage of polysaccharides containing a terminal α-guluronate group, resulting in the formation of oligosaccharides with 4-deoxy-α-erythro-hex-4-enuronosyl groups at their non-reducing ends. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Pseudoalteromonas sp.
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Pseudoalteromonas sp. ulvan lyase 24A can be used for glycobiology research and participates in the eliminative cleavage of ulvan.
