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Recombinant keratanase II from Bacillus circulans
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Cat. No.: X24-08-LY323

Recombinant keratanase II from Bacillus circulans

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Synonym: Keratanase II

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Product Size
1.0 U per vial (10 mU/ul)
Price
Datasheet
Datasheet
MSDS
MSDS
Properties
Description
Recombinant keratanase II from Bacillus circulans cleaves the sulfated N-acetylglucosaminidic β1-3 linkage to galactose included in keratan sulfate.
Unit Definition
One unit will produce 1 umol of reducing oligosaccharides from shark cartilage keratan sulfate polysaccharide per minute at pH 6.0 at 37°C.
Expression System
E. coli
Source
Bacillus circulans
Reaction Conditions
20 mM Sodium acetate, pH 6.0
Production Methods
Fermentation
Detection Method
A 50 ul mixture of recombinant keratanase II and shark cartilage keratan sulfate is incubated at 37°C for 15 min, and the released oligosaccharide reducing end is determined by a modified Somogyi-Nelson method.
Sensitivity
Heat sensitive
Concentration
10 mU/uL
Form
Colorless to Almost colorlessclear liquid
Titer
≥1.0 unit/vial
Buffer
20 mM Sodium phosphate, pH 7.4
Preservative
None
Stabilizer
None
pH
7.4
Applications
Recombinant keratanase II from Bacillus circulans can be used to detect keratin sulfate proteoglycan content.
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