Functions of DSGb5Cer Antigen

Gb5Cer is considered a stage-specific embryonic antigen (SSEA-3) and is also recognized as an important marker of human embryonic stem cells (hESCs). It is commonly associated with the expression of GSL during early embryonic development. DSGb5Cer in Gb5Cer is the major dienophilic ganglioside in renal cell carcinoma (RCC) tissues, and it exhibits high expression in invasive renal cell carcinoma cells and numerous tumor cells. In a previous study, a link between DSGb5Cer expression in renal cell carcinoma tissues and metastasis is found, suggesting that it may be a marker for renal cell carcinoma. In addition, altered DSGb5Cer expression in prostate tissues suggests the progression of malignant potential in prostate cancer. Based on the specific physiological activity of DSGb5Cer, it has emerged as a target for developing tumor-associated vaccines.

Fig.1 Structures of DSGb5 derivatives with different aglycones. (Wu, et al., 2020)

Tumor-Associated DSGb5Cer Antigen Production Service at CD BioGlyco

CD BioGlyco has a specialized Vaccine Development Platform and offers a variety of services regarding vaccine development, such as antigen production. We have mastered several methods in the production of tumor-associated DSGb5Cer antigen.

• Chemo-enzymatic method
  CD BioGlyco relies on chemical reactions on the internal GalNAc unit of the pentasaccharide backbone. Chemical acylation of the GalNAc unit produces a polysaccharide, followed by silylation of the terminal Gal residue using specific enzymes to construct the DSGb5Cer antigen.
  • Advantages: greater flexibility, specificity, and high selectivity.
• Chemical method
  CD BioGlyco offers a polymeric and efficient method of constructing polycycles for the synthesis of DSGb5Cer antigen. We offer different glycosylation methods to synthesize the glycosidic bonds to create optimal conditions for the synthesis of the target product.
  • Advantages: good to excellent yields, excellent stereoselectivity.

In our vaccine development services, we use the DSGb5Cer antigen as the core substance in combination with different surfactants, adjuvants, stabilizers, and other active substances for vaccine development. We offer in vivo and in vitro assays to test the effectiveness of the vaccine.

Fig.2 Synthesis of the DSGb5Cer antigen. (CD BioGlyco)

Applications

• DSGb5Cer antigen is overexpressed in tumor cells and therefore it can be used to detect tumorigenesis.
• DSGb5Cer antigen is used in the development of tumor-related vaccines.
• In pharmaceutical manufacturing, DSGb5Cer antigen can be used in anti-tumor as well as drug development for the treatment of cancer.

Advantages

• We have multiple routes to produce DSGb5Cer antigen with high efficiency and selectivity.
• Every step of our antigen production and vaccine development is quality tested.
• Our research team is specialized and experienced in the field of antigen production to ensure client satisfaction.

CD BioGlyco has established a vaccine research and development platform dedicated to vaccine research and development as well as the production of many types of antigens. Currently, we have accumulated many years of experience in the production of antigens. Among them, for the tumor-related DSGb5Cer antigen, we will provide various methods for its production. Please feel free to contact us if you are interested in our services and have any questions.

Reference

1. Wu, D.Y.; et al. A modular chemoenzymatic synthesis of disialosyl globopentaosylceramide (DSGb5Cer) glycan. J. Org. Chem. 2020, 85(24): 15920-15935.