Functions of Carbohydrate Antigens

Carbohydrates are often displayed in large quantities on the surface of normal and tumor cells in the form of glycoconjugates, such as glycolipids and glycoproteins. Among them, special glycans expressed by cancer cells are known as tumor-associated antigens. It is a useful molecular target for the development of new cancers, tumor therapies, and diagnostics. Among the tumor-associated antigens, the structure of globo-H antigen has a b-GalNAc-(1→3)-a-Gal-(1→4)-Gal oligosaccharide part. It belongs to a hexasaccharide sphingolipid and is present in many tumors in the human body, especially breast, prostate, and lung cancers. Therefore, globo-H has become an important target for the Development of Tumor-Associated Carbohydrate Vaccines.

Fig.1 Structure of tumor-associated globo-H antigen. (Mandal, et al., 2015)

Tumor-Associated Globo-H Antigen Production Service at CD BioGlyco

Globo-H antigen is expressed in many types of tumor cells and plays a key role in the development of anti-tumor vaccines. At CD BioGlyco, we provide our clients the tumor-associated globo-H antigen production services under the general direction of vaccine development.

• Synthesis of globo-H antigen
  • Chemoenzymatic synthesis
    We synthesize globo-H antigen using a rapid assembly-based pre-activated one-pot glycosylation method and combining fucosyltransferase, and sialyltransferase.
  • Glycosylation strategy assembly
    We use different glycosylation methods to assemble different types of glycosidic bonds. The main focus is to utilize specific monosaccharides as glycosyl donors to construct glycosidic bonds and apply them to the synthesis of globo-H antigens.
  • Automated solid phase assembly
    The central step of this method provided by CD BioGlyco is the installation of the r-galactoside linkage. In this method, we carry out purification after the synthesis process is fully completed.
• Vaccine development

Fig.2 Composition of the vaccine. (CD BioGlyco)

Because globo-H is expressed on many tumors in the human body, we also offer the development of anti-tumor vaccines using globo-H as an antigen in combination with substances such as preservatives, stabilizers, surfactants, diluents, and adjuvants.

Fig.3 Antigen synthesis and vaccine development services. (CD BioGlyco)

Applications

• The globo-H antigen can be applied to the development of anti-tumor vaccines.
• In the medical field, globo-H antigen can be applied to explore treatment research for tumors and cancers in humans.
• Globo-H antigen can be applied to detect the occurrence of tumors or cancers.

Advantages

• We have a professional vaccine development and production platform and are quite skilled in the production of globo-H antigens.
• Each step of our production is quality tested to ensure client satisfaction.

CD BioGlyco has extensive experience in the Production of Tumor-Associated Globo Protein Antigens and can provide clients with a wide range of antigen types as well as vaccine development services. Currently, we have mastered the production of the tumor-associated globo-H antigen. Please feel free to contact us if you are interested in our services and have any questions.

References

1. Mandal, S.S.; et al. Chemical synthesis of the tumor-associated globo H antigen. RSC Advances. 2015, 5: 23311-23319.
2. Wang, Z.; et al. Chemoenzymatic syntheses of tumor-associated carbohydrate antigen globo-H and stage-specific embryonic antigen 4. Adv Synth Catal. 2008, 350: 1717-1728.