Biological Background of Acetoacetyl Coenzyme A (Acetoacetyl CoA)

Acetoacetyl CoA is the precursor of the synthesis of β-hydroxy-β-methylglutarate monoacyl CoA (HMG-CoA) through the mevalonate pathway, which is controlled by HMG-CoA synthase and is essential for cholesterol synthesis. It also plays a role in the biosynthesis of ketone bodies in the liver, where the process begins with two Acetyl-CoA molecules that condense to acetoacetyl CoA under the action of acetoacetyl-CoA thiolase to. In addition, phosphotransferase (PTB) and poly-3-hydroxybutyrate (PHB) can be biosynthesized from acetoacetyl CoA, and PHB has a high application prospect.

Fig.1 Acetoacetyl CoA in the biosynthesis of ketone bodies. (CD BioGlyco)

How to Prepare Acetoacetyl CoA

Enzyme-catalyzed biosynthesis strategy is adopted. The gene encoding acetoacetyl-CoA synthetase from the streptomyces strain is recombined and overexpressed into Escherichia coli, and the target product is prepared by fermentation followed by purification.

Acetoacetyl Coenzyme A Production Service at CD BioGlyco

CD BioGlyco provides acetoacetyl CoA in solid form. Before the application, the aqueous solution can be prepared by solving acetoacetyl CoA solid directly into phosphate-buffered saline (PSB). It is recommended to use the solution immediately, and the storage time should not exceed one day.

• Acetoacetyl CoA in isoprenoid pathway: It is produced by the condensation of two acetyl-CoA molecules catalyzed by thiolase. As a precursor of HMG-CoA, acetoacetyl CoA plays an important role in cholesterol biosynthesis.
• Acetoacetyl CoA in ketone bodies synthesizing: It is a key intermediate in ketone body biosynthesizing in the liver. When the body is deficient in glucose, ketone bodies work as an important energy source for extra-hepatic tissues such as the heart, kidney, skeletal muscle, and even the brain.
• Acetoacetyl CoA in PHB synthesizing: PHB is a degradable polymer existing in nature, with excellent biocompatibility as a novel functional material. PHB has great application value in various fields including industry, food, environmental protection, and biomedical technology. Acetoacetyl CoA is a vital intermediate in microbial PHB synthesis. Thanks to unremitting research, the biosynthesis of PHB can be achieved by constructing genetically engineered bacteria and transgenic plants.

Applications

• Study on the mechanism of fat and cholesterol biosynthesis.
• Study on synthesis and metabolism of ketone bodies.
• Preparation of novel functional material, PHB.

Highlights

• Acetoacetyl CoA is solid with stable properties.
• Mature microbial fermentation strategy.
• Safe and efficient production technology.
• Comprehensive laboratory and production platform.

CD BioGlyco has a large team of scientists and state-of-the-art Platforms. It is our mission to provide you with custom solutions and quality services in the development and production of biotechnology and therapies for diseases. If you are interested in our Custom Sugar-nucleotides Synthesis service, please feel free to contact us now.