Bioactivity of Acetyl Coenzyme A (Ac-CoA)

Ac-CoA is a membrane-impervious central metabolic intermediate, the actual form for pyruvate entering the tricarboxylic acid cycle (TCA cycle) during glycolysis, a vital precursor of lipid synthesis, the unique donor of acetyl acetylation, and an effective allosteric activator of pyruvate carboxylase. In addition, it is also a source of the neurotransmitter acetylcholine. In short, Ac-CoA has indispensable importance in living organisms.

Synthetic Strategies

Ac-CoA can be conventionally obtained by chemical synthesis using acetic anhydride and CoA as raw materials. Enzyme catalysis strategy and biosynthesis strategy are used more frequently. Ac-CoA can be synthesized from 1-acetylimidazole and CoA by the metabolism of Bacillus subtilis. It can be obtained from sodium pyruvate and CoA by the action of bacterial pyruvate dehydrogenase complex. If Acetoacetyl-CoA is available, it is a very rapid method to break down acetoacetyl CoA into Ac-CoA by thiolysis. In the presence of acetate CoA-transferase, Ac-CoA can be converted from the reaction of acetic acid and succinyl-CoA.

Fig.1 Different synthetic strategies for Ac-CoA production. (CD BioGlyco)

Acetyl Coenzyme A Production Service at CD BioGlyco

Ac-CoA plays an important role in vivo and has a promising application in the research field. CD BioGlyco produces high-quality products to support your applications in a variety of studies.

• Ac-CoA in acetyl transfer reaction: Ac-CoA is a necessary carrier and cofactor of acetyl group in enzyme-catalyzed acetyl transfer reactions. During the post-translational modification of proteins, it provides acetyl groups to target amino acid residues for the regulation of various cellular activities.
• Ac-CoA in autophagy: Ac-CoA can increase protein acetylation in hungry cells and decrease hunger-induced autophagy flux. In vivo experiments have shown that Ac-CoA alleviates cardiomyopathy induced by stress overload in mice by inhibiting undesirable autophagy.
• Ac-CoA in assays of chloramphenicol acetyltransferase (CAT) activity: Ac-CoA is used to test CAT activity in cell extracts by radioisotopes on the principle that acetyl groups can be catalyzed by CAT and transfer to chloramphenicol from Ac-CoA.
• Ac-CoA in assays of citrate synthase activity: In the TCA cycle, citrate is formed by the condensation of oxaloacetic acid and Ac-CoA catalyzed by citrate synthase. Ac-CoA can be used as a substrate and energy donor to test citrate synthase activity.

Applications

• Development of cardiovascular disease therapy.
• Exploring the regulatory mechanism of autophagy.
• Study on post-translational acetylation of proteins.
• CAT activity assay.
• Citrate synthase activity assay.

Highlights

• Sophisticated chemical synthesis method for Ac-CoA production.
• Optional enzymatic synthesis methods.
• Environmentally friendly biotransformation strategies.
• Experienced technicians.
• Platforms with advanced technology.

CD BioGlyco adheres to the concept of producing excellent products and serving customers with heart. We provide custom solutions to help you solve any challenge. If you are interested in our acetyl coenzyme A production service and other Nucleotide-based Production Services, please feel free to contact our experts to provide you with professional insights.