Chicken lipopolysaccharide-binding protein (LBP) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of chicken LBP as low as 1 ng/mL and its detection range is 6.25-200 ng/mL.
Assay Principle
Chicken lipopolysaccharide-binding protein (LBP) ELISA kit is used to detect the level of Chicken LBP in a sample. It involves coating microtiter plate wells with purified chicken LBP antibody to create a solid-phase antibody. LBP is then added to the wells. Both the LBP and the antibody are labeled with HRP, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added. The TMB substrate turns blue under the catalysis of HRP enzyme. The reaction is stopped by adding a sulphuric acid solution, and the color change is measured using a spectrophotometer at a wavelength of 450 nm. By comparing the optical density (O.D.) of the samples to a standard curve, the concentration of LBP in the samples can be determined.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Assay Target
LBP
Species Reactivity
Chicken
Components
MicroELISA plate; Enzyme conjugate; Standard A; Standard B; Standard C; Standard D; Standard E; Standard F; Substrate A; Substrate B; Stop solution; Wash solution; Balance solution; Instruction
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
6.25-200 ng/mL
Sensitivity
1 ng/mL
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Chicken lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of chicken LBP in serum, plasma, tissue homogenate and other biological fluid samples.
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