Human lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of human LPS as low as 0.1 pg/mL and its detection range is 0.5-16 pg/mL.
Assay Principle
Human lipopolysaccharide (LPS) ELISA kit is specifically designed for measuring the level of human LPS in a given sample. To perform the assay, purified human LPS antibodies are used to coat the wells of a microtiter plate, creating a solid-phase antibody. Next, the LPS sample is added to the wells, resulting in the binding of LPS molecules to the antibodies. The antibodies are labeled with HRP, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added to the wells, causing the TMB substrate to undergo an HRP enzyme-catalyzed reaction, leading to a color change to blue. To terminate the reaction, a sulphuric acid solution is added, and the resulting color change is measured using a spectrophotometer at a wavelength of 450 nm. Finally, the concentration of Porcine LPS in the samples can be determined by comparing the optical density (O.D.) of the samples to a standard curve.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
0.5-16 pg/mL
Sensitivity
0.1 pg/mL
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Human lipopolysaccharide (LPS) ELISA kit can be used to measure the level of human LPS in serum, plasma, tissue homogenate and other biological fluid samples.
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