Mouse lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of mouse LPS as low as 1 EU/L and its detection range is 1-32 EU/L.
Assay Principle
Mouse lipopolysaccharide (LPS) ELISA kit is designed to measure the level of Mouse LPS (lipopolysaccharide) in a sample. It utilizes purified Mouse LPS antibodies that are immobilized on the wells of a microtiter plate, creating a solid-phase antibody. The sample containing LPS is then added to the wells, and the LPS molecules bind to the immobilized antibodies, forming an antibody-antigen complex. This complex is detected by adding HRP-labeled antibodies that specifically recognize the LPS. After thorough washing to remove any unbound components, a TMB substrate solution is added. In the presence of HRP, TMB substrate undergoes a color change to blue. The reaction is stopped by adding a sulfuric acid solution, resulting in a color change that can be measured using a spectrophotometer at a wavelength of 450 nm. The concentration of LPS in the sample can be determined by comparing the optical density (O.D.) of the sample to a standard curve.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
1-32 EU/L
Sensitivity
1 EU/L
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Applications
Mouse lipopolysaccharide (LPS) ELISA kit can be used to measure the level of mouse LPS in serum, plasma, tissue homogenate and other biological fluid samples.
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