Nicotinamide Adenine Dinucleotide Phosphate (NADP) Production Service
The Biological Properties of the NADP
NADP is a coenzyme bound to a phosphate molecule in an ester bond, widely found in living organisms. NADP and its reduced form NADPH regulate reductive metabolism in a subcellular segregated manner and are central cofactors in lipid and redox homeostasis. NADPH is expressed in the reduced form through several enzymes in the cell and is recycled in the reduced NADPH and oxidized states NADP. NADP production in mitochondria is dependent on the phosphorylation of NAD (H) by NAD kinase 2 (NADK2), which is used to power biosynthetic pathways (including lipid synthesis) and redox homeostasis. NADK2 is important in the regulation of cellular redox state, energy metabolism, mitochondrial function, gene expression, and signaling pathways. NADK2-mediated mitochondrial NADP production is required for glutamate reduction and proline biosynthesis for cytoplasmic protein synthesis.
Fig.1 NADP biosynthesis pathway in mitochondria. (Zhu, et al., 2021)
NADP Production Service at CD BioGlyco
CD BioGlyco provides high-quality, high-purity NADP production services. we have professional experimental personnel to follow up at any time on, the rigorous production process, to provide customers with the best quality service. About the production of NADP, we mainly provide the following services：
- Whole-cell catalytic method
The method of NADP generation by whole-cell catalysis developed by our company is safe, efficient, and controllable. Reducing the energy consumption and material consumption of the process. We strictly control the whole process, and constantly optimize the catalytic conditions to ensure the quality of our products.
- Chemical synthesis
CD BioGlyco uses the coupling reaction of benzene ethanol phosphate monoester and NMN as the basic model of the reaction synthesis route. Process optimization by parameters such as reaction time and temperature. Conversion of NAD to NADP by a phosphorylation reaction.
- De novo method
CD BioGlyco provides ab initio NADP synthesis catalyzed by two NADK isoforms, a cytoplasmic NADK, and a mitochondrial NADK2. NADP is synthesized abinitio in cells by a single enzymatic reaction that adds the phosphate portion of a donor from a nucleotide, usually ATP, to the 2' position of NAD+. NADP is efficiently synthesized by this.
- New technologies
The engineered NADK producer was genetically engineered using a plasmid, fixed to submicron particles after overexpression, and used to catalyze NAD for NADP production in vitro.
Fig.2 Schematic diagram of de novo method. (CD BioGlyco)
Applications
- Cellular level: Metabolic reprogramming and metabolic plasticity for NADP allow cancer cells to fine-tune their metabolism and adapt to the ever-changing environments of the metastatic cascade. Such as, it can be used as a therapeutic target for metastatic breast cancer.
- NADP is useful for the synthesis of the cytosolic proteins.
- NADP-coupled nanoparticles play a role in biological functional analysis.
As a professional Carbohydrate Synthesis company, CD BioGlyco provides high-purity Nucleotide-based Production services to customers all over the world. In addition, we provide efficient cAMP, cGMP, FAD, FMN, NAD, and Guanosine Pentaphosphate (pppGpp) Production Services. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening project time. If you are interested in our service, please feel free to contact us further to introduce the specific service in detail.
References
- Zhu, J.; et al. Mitochondrial NADP(H) generation is essential for proline biosynthesis. Science. 2021, 372(6545): 968-972.
- Hoxhaj, G.; et al. Direct stimulation of NADP+ synthesis through Akt-mediated phosphorylation of NAD kinase. Science. 2019, 363(6431): 1088-1092.
