The Functions of cGMP

cGMP is distributed in different cells and tissues. cGMP targets a wide range of downstream effector molecules, thereby eliciting a very wide range of multiple cellular effects. It is induced by the stimulation of either soluble guanylyl cyclase (sGC) or granular guanylyl cyclase (pGC), both enzymes are present in different isoforms. Due to the distribution of sGC and PGC and the process of ligand formation, the cGMP pathway not only regulates the cardiovascular system, but also renal, pulmonary, hepatic, and cerebral functions. In addition, this pathway is implicated in the development of neurological lesions and inflammation and also plays a role in some infectious diseases.

Fig.1 Schematic representation of the main cGMP drug intervention sites. (Friebe, et al., 2020)

cGMP Production Service at CD BioGlyco

According to the different demands of clients, CD BioGlyco provides high-purity cGMP production services through several efficient extraction and detection processes. We have accumulated many years of successful experience in synthesis and analysis. Our company constantly optimizes the production process, and development of new production extraction processes. We provide the following production services:

• Chemical synthesis

We synthesize useful organic molecules under mild reaction conditions through a copper-catalyzed azoreyne cycloaddition reaction (CuAAC). CuAAC is highly attractive for the modification of complex and sensitive molecules such as nucleotides. Here, we carry out the CuAAC reaction by reacting the 3'-O propargyl guanosine monophosphate with alkyl bromide using water as a solvent.

• Natural products extraction

• Extraction technology: the extraction methods we provide for cGMP from natural products include solvent extraction, physically assisted extraction, and enzymatic method.

• The solvent extraction services we provide include thermal water immersion formulation and ethanol extraction method. optimize optimization according to the products of different sources, and select the extraction method with the highest yield.
• The physical auxiliary method is mainly extracted by microwave-assisted extraction and ultrasonic assistance, the yield reaches 300 μg/g.
• The enzymatic method we mainly according to your product requirements, and the targeted selection of enzyme-specific and efficient cGMP production.

• New technologies

We continue to innovate, research, and develop new extraction technology, including membrane separation technology, supercritical fluid extraction technology, high-speed reverse current chromatography separation technology, and other new extraction methods.

• Detection service

CD BioGlyco also provides a post-production detection service for cGMP. We conduct a quantitative and qualitative analysis of cGMP by protein binding, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), enzyme-linked immune analysis, liquid chromatography-tandem mass spectrometry (LC-MS), and other methods.

Fig.2 The production services of cGMP. (CD BioGlyco)

Applications of cGMP

• cGMP can be used in fibrotic remodeling.
• cGMP plays a key role in neuronal function and the screening of CNS diseases.
• Physiology and drug therapy: a powerful tool of cGMP coupled biosensors for sensitivity analysis at the single cell level.

Advantages

• Detection service offered by our company with high sensitivity, qualitative and quantitative accuracy.
• Each step of the process extraction was strictly controlled.

CD BioGlyco has perfect experimental equipment and professional technical personnel, to provide customers with high-quality Nucleotide-based Production services. Specifically, we provide cAMP, FAD, FMN, NAD, NADP, Guanosine Pentaphosphate (pppGpp) Production Services, and so on. Our advanced technology and exceptional research team are well-equipped to fulfill your requirements. If you want to know more, please feel free to contact us.