Non-natural Fuc Analog-based Cell Surface Metabolic Oligosaccharide Engineering (MOE) in Bio-pharmaceuticals

Non-natural Fuc analog-based cell surface MOE is based on the principle of modifying cellular interactions with other cells or molecules by introducing non-natural Fuc analogs that modulate the structure of the glycan groups on the cell surface. This opens up the possibility of designing and optimizing targeted immunotherapies. For example, enhancement of anti-tumor immune responses by enhancing the interaction between immune cells and tumor cells. This technology can also be used in the discovery and development of innovative drugs. While many current drugs are designed based on the principle that specific molecules interact with cell surface glycan structures, the introduction of non-natural Fuc analogs can precisely modulate cell surface glycan structures, thereby altering the target or mode of action of the drug and improving drug efficacy and specificity.

Fig.1 Strategies of MOE. (Campbell, et al., 2007)

Non-natural Fuc Analog-based Cell Surface MOE Service at CD BioGlyco

CD BioGlyco provides non-natural Fuc analog-based Cell Surface MOE Services with superior technology and a specialized Glycoengineering Platform. We use non-natural Fuc analogs to modification of glycosylation on the cell surface to achieve precise modulation of specific protein-glycan interactions. Below are details about our services:

• Firstly, we synthesize a variety of non-natural Fuc analogs by chemical synthesis or enzymatic methods and screen these analogs to determine the optimal synthesis protocol. At the same time, key enzyme genes required for the synthesis of non-natural Fuc analogs will be inserted into the target strains by genetic engineering techniques.
• Subsequently, we modulated metabolic pathways in the constructed genetically engineered strains to promote the biosynthesis of unnatural Fuc analogs. We optimized the culture conditions such as temperature, pH, and nutrients during the culture process to enhance the ability of the cells to produce the target non-natural Fuc analogs.
• Finally, we isolate, purify, and characterize the target unnatural Fuc analogs from the cultures.

Fig.2 Applications of non-natural Fuc analog-based cell surface MOE. (CD BioGlyco)

Advantages of Us

• Introducing non-natural Fuc analogs through synthetic chemistry allows for precise modulation, helping you to synthesize your target products more accurately.
• Non-natural Fuc analogs are well-stabilized on the cell surface and do not significantly interfere with or are toxic to the normal functional and physiological processes of your cell samples.
• Our team provides detailed reports of experiments based on your needs and goals and scientifically interprets and analyzes the results.

CD BioGlyco offers non-natural Fuc analogs-based cell surface MOE services to help clients achieve precise Cell Surface Glycoengineering for various research and application needs. We aim to provide valuable assistance to researchers and scientists in their studies on glycobiology. Please feel free to contact us if you have any questions regarding our services.