Structure of Deoxyguanosine

Deoxyguanosine pertains to organic compounds called purine 2'-deoxyribonucleosides. The base guanine and the deoxyribose are the basic composition of deoxyguanosine. Deoxyguanosine is similar to guanosine in chemical structure, but the difference is 2' position of the ribose sugar was taken out of the hydroxyl group. Deoxyguanosine monophosphate attached a phosphate group at the 5' position.

Fig.1 Structure of deoxyguanosine. (Wikipedia)

Properties of Deoxyguanosine

Biologically, deoxyguanosine plays a fundamental role in the genetic code as one of the four deoxyribonucleosides that constitute DNA. It commonly exists in all living species, including bacteria, plants, animals, and humans. At the same time, lots of enzymatic reactions need the participation of deoxyguanosine. As an essential component of DNA, it plays a crucial role in genetic information storage and transfer. Biologically, it pairs with cytosine (C) through three hydrogen bonds, forming one of the complementary base pairs that make up the DNA double helix structure.

Fig.2 Pathways of deoxyguanosine metabolism in human T-cells. (Basso, et al., 2005)

Nucleoside-based Deoxyguanosine Production Service at CD BioGlyco

CD BioGlyco has advanced equipment, technologies, and highly experienced staff to provide one-stop service in terms of deoxyguanosine production services for clients.

• Production methods
  
  • Chemical synthesis
    
    • The solid-supported phosphoramidite method.
  • Biosynthetic methods
    
    • The metabolically engineered Escherichia coli strain can be applied to produce deoxyguanosine with a relatively high yield.
• Quality control analysis
  CD BioGlyco performs the most widely used methods for analysis, including:
  
  • Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
    
    • To determine expression products.
  • High-performance liquid chromatography (HPLC), mass spectrometry (MS)
    
    • To identify and quantify the nucleoside content and purity.
• Production capacity
  
  • Large-scale production.
• Structure modification
  
  • Modification of deoxyguanosine structure according to the specific needs of clients' research and project development to obtain products with different uses and functions.

Fig.3 Nucleoside-based deoxyguanosine production. (CD BioGlyco)

Applications

• Used in molecular biology techniques
  
  • Widely used in research for DNA sequencing, polymerase chain reaction (PCR), DNA labeling, and other molecular biology techniques.
• Used as the precursors
  
  • Serves as a precursor for the synthesis of modified nucleotides and nucleic acid analogs.
• Used as the substrate
  
  • The enzymes that participated in DNA repair processes use deoxyguanosine as an essential substrate. These enzymes recognize and remove damaged or mismatched bases from DNA, contributing to the maintenance of genome integrity.

Advantages

• The production process is flexible and can be adjusted according to actual needs.
• The production process helps maintain the stability and yield of the deoxyguanosine.
• All operations can be completed in a closed system with less pollution and is environmentally friendly.

CD BioGlyco offers professional after-sale services to achieve customer satisfaction. Please feel free to contact us for a consultation for Nucleoside Production Services.