Rat lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of rat LPS as low as 1 EU/L and its detection range is 25-800 EU/L.
Assay Principle
Rat lipopolysaccharide (LPS) ELISA kit is designed to measure rat LPS levels in samples using a microtiter plate coated with purified rat LPS antibody to create a solid-phase antibody. LPS is added to the wells, followed by the addition of HRP-labeled antibody, resulting in the formation of an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added, leading to the development of a blue color as a result of HRP enzyme catalysis. The reaction is stopped by adding a sulfuric acid solution, and the change in color is measured at a wavelength of 450 nm using a spectrophotometer. The concentration of LPS in the samples is determined by comparing the Optical Density (O.D.) of the samples to a standard curve.
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
25-800 EU/L
Sensitivity
1 EU/L
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Recovery
>95%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Rat lipopolysaccharide (LPS) ELISA kit can be used to measure the level of rat LPS in serum, plasma, tissue homogenate and other biological fluid samples.
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