Properties
Description
Recombinant Bacteroides thetaiotaomicron xylose isomerase A produced by Escherichia coli, functions as an enzyme produced in Pichia pastoris. It facilitates the oxidation of cellobiose to cellobiose-1,5-lactone.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Xylose isomerase
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron xylose isomerase A can be used for glycobiology research. It has the ability to hydrolyze D-xylose and D-xylulose.