Properties
Description
Recombinant Enterococcus faecalis lytic cellulose monooxygenase 10A produced by Escherichia coli, is classified as a copper-dependent lytic polysaccharide monooxygenase (LPMO) and is assigned the E.C. number 1.14.99.54.
Expression System
Escherichia coli
Species
Enterococcus faecalis
Purity
≥90%, determined by SDS-PAGE.
Activity
Lytic polysaccharide monooxygenases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Enterococcus faecalis lytic cellulose monooxygenase 10A can be used in the biorefinery industry and participates in the eliminative cleavage of chitin, chitooligosaccharides.