Functions of Metabolic Oligosaccharide Engineering

Metabolic oligosaccharide engineering is an emerging technology that enables the exogenous provision of unnatural monosaccharide analogs to living cells and their incorporation into cell surface glycans by biosynthesis. For example, metabolic oligosaccharide engineering enables utilizing unnatural GlcNAc and its analogs ManNAc and GalNAc to modulate selectin-mediated adhesion linked to leukocyte extravasation and cancer cell metastasis, as they also target GlcNAc analogs with functionalized probes to various tissues for drug delivery. Currently, unnatural sugars containing biogenic amylose moieties are widely used to help label glycosylated proteins for cellular imaging of glycoproteins, tandem enrichment, and use in proteomics analysis. In addition, glycosylation labeling can be used to identify additional glycosylation sites, thus enabling glycosylation of the cell surface.

Fig.1 Strategies for metabolic oligosaccharide engineering. (Campbell, et al., 2007)

Non-natural GlcNAc Analog-based Cell Surface Metabolic Oligosaccharide Engineering Service at CD BioGlyco

At CD BioGlyco, based on Cell Surface Metabolic Oligosaccharides Engineering techniques and research of metabolic pathways, we offer our clients unnatural GlcNAc analogs-based cell surface metabolic oligosaccharides engineering services.

• Cell surface function modulation
  We utilize non-natural GlcNAc analog metabolic oligosaccharide labeling to attach functionalization groups to the cell surface. By adding polymers with orthogonal reactivity, the azide group can be used as an anchor point for fast, simple, and powerful "click" functionalization of the cell surface. This is an effective way to achieve payload attachment and modulation of cellular functions.
• Glycoprotein modification
  We incorporate unnatural forms of GlcNAc analogs into glycoconjugates that appear on the cell surface and modify O-GlcNAc proteins involved in intracellular signaling. We uniquely modify cell surface glycosylation sites by specific repeating oligosaccharides to enable cellular imaging or selective enrichment of glycoproteins.

Fig.2 Cell surface metabolic oligosaccharides engineering services based on unnatural GlcNAc analogs. (CD BioGlyco)

Applications

• Cell surface metabolic oligosaccharide engineering can be used to probe host-pathogen interactions.
• Non-natural oligosaccharide-based cell surface metabolism oligosaccharide engineering can be used in selective antibody and immunorecognition studies in cancer therapy.
• Non-natural oligosaccharide-based cell surface metabolic oligosaccharide engineering can be used in drug delivery studies.

Advantages

• Our cell surface metabolic oligosaccharide engineering technique promises to develop immune targeting strategies for different pathogens.
• We provide cell surface metabolic oligosaccharide engineering that helps scientists probe deeply into the mechanism of cell surface glycosylation.

CD BioGlyco has established a Glycoengineering Platform relying on specialized equipment to provide different glycoengineering technique services. For example, in terms of cell surface glycoengineering, we provide Metabolic Pathway-based Cell Surface Glycoengineering Services, Chemical Conjugation-based Cell Surface Glycoengineering Services, and other services. We are confident to be your right hand in the field of glycoengineering. If you are interested in our services, please feel free to contact us.